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一.產品名稱：Recombinant Human TNF-α/Peprotech  TNF-α

二.Catalog Number:AF-300-01A

三.Synonyms:

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

四.Description:

TNF-α is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Manufactured using all non-animal reagents.

五.AA Sequence:

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

The ED₅₀as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

九.Cross Reactivity:Cow, Monkey, Mouse, Rat, Sheep, Human, Dog, Pig, N/A, Human + Virus